rabbit anti prm1 183 prm2 transition protein 1 Search Results


spag8  (Proteintech)
92
Proteintech spag8
Spag8, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+prm1+183+prm2+transition+protein+1/pmc09270976-390-18-19?v=Proteintech
Average 92 stars, based on 1 article reviews
spag8 - by Bioz Stars, 2026-07
92/100 stars
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odf2  (Proteintech)
93
Proteintech odf2
(A) CMA3 staining of mature WT and dHET sperm counterstained by DAPI. Scale bars: 30 μm. (B) Quantification of CMA3-stained nuclei in WT and dHET samples. Data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (***p<0.001). (C) Representative image of a Coomassie stained AU-PAGE of basic nuclear-enriched proteins isolated from WT and dHET epididymal sperm. Bands corresponding to <t>ODF2</t> and protamines are marked. Bands containing predominantly PRM1 and mature PRM2 (mP2) are labelled and areas of bands containing Prm2 precursors are marked (red dashed box). Non-protamine, nuclear-enriched proteins are found to be differentially abundant in dHET compared to WT sperm (red box). (D) Upper part of image shown in (C) compared to merged Western Blots against ODF2, histone H3 and histone H4 (pan-H3 and pan-H4 antibodies). (E) Western blots against histone H3 and H4 basic protein extractions of WT testis lysate and sperm samples form WT, dHET, Prm1 +/- (P1 +/- ), Prm1 -/- (P1 -/- ), Prm2 +/- (P2 +/- ), Prm2 -/- (P2 -/- ). ODF2 was used as loading control, as described before . (F) IHC against pre-PRM2 (antibody epitope in cP2) on WT and dHET epididymal caput tissue sections. (G) IHC against transition proteins TNP1 and TNP2 on WT and dHET epididymal caput tissue sections. Scales (F-G): 50 µm
Odf2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+prm1+183+prm2+transition+protein+1/bio_rxiv__64898__2026__03__15__711850-117-29-30?v=Proteintech
Average 93 stars, based on 1 article reviews
odf2 - by Bioz Stars, 2026-07
93/100 stars
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rabbit anti-phh3 #06-570  (Merck KGaA)
90
Merck KGaA rabbit anti-phh3 #06-570
(A) CMA3 staining of mature WT and dHET sperm counterstained by DAPI. Scale bars: 30 μm. (B) Quantification of CMA3-stained nuclei in WT and dHET samples. Data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (***p<0.001). (C) Representative image of a Coomassie stained AU-PAGE of basic nuclear-enriched proteins isolated from WT and dHET epididymal sperm. Bands corresponding to <t>ODF2</t> and protamines are marked. Bands containing predominantly PRM1 and mature PRM2 (mP2) are labelled and areas of bands containing Prm2 precursors are marked (red dashed box). Non-protamine, nuclear-enriched proteins are found to be differentially abundant in dHET compared to WT sperm (red box). (D) Upper part of image shown in (C) compared to merged Western Blots against ODF2, histone H3 and histone H4 (pan-H3 and pan-H4 antibodies). (E) Western blots against histone H3 and H4 basic protein extractions of WT testis lysate and sperm samples form WT, dHET, Prm1 +/- (P1 +/- ), Prm1 -/- (P1 -/- ), Prm2 +/- (P2 +/- ), Prm2 -/- (P2 -/- ). ODF2 was used as loading control, as described before . (F) IHC against pre-PRM2 (antibody epitope in cP2) on WT and dHET epididymal caput tissue sections. (G) IHC against transition proteins TNP1 and TNP2 on WT and dHET epididymal caput tissue sections. Scales (F-G): 50 µm
Rabbit Anti Phh3 #06 570, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+prm1+183+prm2+transition+protein+1/pmc05504044__41598_2017_5286_MOESM1_ESM-8-22-23?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
rabbit anti-phh3 #06-570 - by Bioz Stars, 2026-07
90/100 stars
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alexa fluor  (Jackson Immuno)
96
Jackson Immuno alexa fluor
(A) CMA3 staining of mature WT and dHET sperm counterstained by DAPI. Scale bars: 30 μm. (B) Quantification of CMA3-stained nuclei in WT and dHET samples. Data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (***p<0.001). (C) Representative image of a Coomassie stained AU-PAGE of basic nuclear-enriched proteins isolated from WT and dHET epididymal sperm. Bands corresponding to <t>ODF2</t> and protamines are marked. Bands containing predominantly PRM1 and mature PRM2 (mP2) are labelled and areas of bands containing Prm2 precursors are marked (red dashed box). Non-protamine, nuclear-enriched proteins are found to be differentially abundant in dHET compared to WT sperm (red box). (D) Upper part of image shown in (C) compared to merged Western Blots against ODF2, histone H3 and histone H4 (pan-H3 and pan-H4 antibodies). (E) Western blots against histone H3 and H4 basic protein extractions of WT testis lysate and sperm samples form WT, dHET, Prm1 +/- (P1 +/- ), Prm1 -/- (P1 -/- ), Prm2 +/- (P2 +/- ), Prm2 -/- (P2 -/- ). ODF2 was used as loading control, as described before . (F) IHC against pre-PRM2 (antibody epitope in cP2) on WT and dHET epididymal caput tissue sections. (G) IHC against transition proteins TNP1 and TNP2 on WT and dHET epididymal caput tissue sections. Scales (F-G): 50 µm
Alexa Fluor, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+prm1+183+prm2+transition+protein+1/pmc05504044__41598_2017_5286_MOESM1_ESM-8-41-37?v=Jackson+Immuno
Average 96 stars, based on 1 article reviews
alexa fluor - by Bioz Stars, 2026-07
96/100 stars
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anti tnp1  (Proteintech)
93
Proteintech anti tnp1
(A) CMA3 staining of mature WT and dHET sperm counterstained by DAPI. Scale bars: 30 μm. (B) Quantification of CMA3-stained nuclei in WT and dHET samples. Data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (***p<0.001). (C) Representative image of a Coomassie stained AU-PAGE of basic nuclear-enriched proteins isolated from WT and dHET epididymal sperm. Bands corresponding to <t>ODF2</t> and protamines are marked. Bands containing predominantly PRM1 and mature PRM2 (mP2) are labelled and areas of bands containing Prm2 precursors are marked (red dashed box). Non-protamine, nuclear-enriched proteins are found to be differentially abundant in dHET compared to WT sperm (red box). (D) Upper part of image shown in (C) compared to merged Western Blots against ODF2, histone H3 and histone H4 (pan-H3 and pan-H4 antibodies). (E) Western blots against histone H3 and H4 basic protein extractions of WT testis lysate and sperm samples form WT, dHET, Prm1 +/- (P1 +/- ), Prm1 -/- (P1 -/- ), Prm2 +/- (P2 +/- ), Prm2 -/- (P2 -/- ). ODF2 was used as loading control, as described before . (F) IHC against pre-PRM2 (antibody epitope in cP2) on WT and dHET epididymal caput tissue sections. (G) IHC against transition proteins TNP1 and TNP2 on WT and dHET epididymal caput tissue sections. Scales (F-G): 50 µm
Anti Tnp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+prm1+183+prm2+transition+protein+1/pm32726616-302-155-161?v=Proteintech
Average 93 stars, based on 1 article reviews
anti tnp1 - by Bioz Stars, 2026-07
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mouse anti vegf a  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse anti vegf a
(A) CMA3 staining of mature WT and dHET sperm counterstained by DAPI. Scale bars: 30 μm. (B) Quantification of CMA3-stained nuclei in WT and dHET samples. Data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (***p<0.001). (C) Representative image of a Coomassie stained AU-PAGE of basic nuclear-enriched proteins isolated from WT and dHET epididymal sperm. Bands corresponding to <t>ODF2</t> and protamines are marked. Bands containing predominantly PRM1 and mature PRM2 (mP2) are labelled and areas of bands containing Prm2 precursors are marked (red dashed box). Non-protamine, nuclear-enriched proteins are found to be differentially abundant in dHET compared to WT sperm (red box). (D) Upper part of image shown in (C) compared to merged Western Blots against ODF2, histone H3 and histone H4 (pan-H3 and pan-H4 antibodies). (E) Western blots against histone H3 and H4 basic protein extractions of WT testis lysate and sperm samples form WT, dHET, Prm1 +/- (P1 +/- ), Prm1 -/- (P1 -/- ), Prm2 +/- (P2 +/- ), Prm2 -/- (P2 -/- ). ODF2 was used as loading control, as described before . (F) IHC against pre-PRM2 (antibody epitope in cP2) on WT and dHET epididymal caput tissue sections. (G) IHC against transition proteins TNP1 and TNP2 on WT and dHET epididymal caput tissue sections. Scales (F-G): 50 µm
Mouse Anti Vegf A, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+prm1+183+prm2+transition+protein+1/pmc05504044__41598_2017_5286_MOESM1_ESM-8-10-13?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
mouse anti vegf a - by Bioz Stars, 2026-07
96/100 stars
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rabbit anti prm1 183 prm2 transition protein 1  (Proteintech)
93
Proteintech rabbit anti prm1 183 prm2 transition protein 1
(A) CMA3 staining of mature WT and dHET sperm counterstained by DAPI. Scale bars: 30 μm. (B) Quantification of CMA3-stained nuclei in WT and dHET samples. Data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (***p<0.001). (C) Representative image of a Coomassie stained AU-PAGE of basic nuclear-enriched proteins isolated from WT and dHET epididymal sperm. Bands corresponding to <t>ODF2</t> and protamines are marked. Bands containing predominantly PRM1 and mature PRM2 (mP2) are labelled and areas of bands containing Prm2 precursors are marked (red dashed box). Non-protamine, nuclear-enriched proteins are found to be differentially abundant in dHET compared to WT sperm (red box). (D) Upper part of image shown in (C) compared to merged Western Blots against ODF2, histone H3 and histone H4 (pan-H3 and pan-H4 antibodies). (E) Western blots against histone H3 and H4 basic protein extractions of WT testis lysate and sperm samples form WT, dHET, Prm1 +/- (P1 +/- ), Prm1 -/- (P1 -/- ), Prm2 +/- (P2 +/- ), Prm2 -/- (P2 -/- ). ODF2 was used as loading control, as described before . (F) IHC against pre-PRM2 (antibody epitope in cP2) on WT and dHET epididymal caput tissue sections. (G) IHC against transition proteins TNP1 and TNP2 on WT and dHET epididymal caput tissue sections. Scales (F-G): 50 µm
Rabbit Anti Prm1 183 Prm2 Transition Protein 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+prm1+183+prm2+transition+protein+1/pm32554096-62-65-71?v=Proteintech
Average 93 stars, based on 1 article reviews
rabbit anti prm1 183 prm2 transition protein 1 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

Image Search Results


(A) CMA3 staining of mature WT and dHET sperm counterstained by DAPI. Scale bars: 30 μm. (B) Quantification of CMA3-stained nuclei in WT and dHET samples. Data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (***p<0.001). (C) Representative image of a Coomassie stained AU-PAGE of basic nuclear-enriched proteins isolated from WT and dHET epididymal sperm. Bands corresponding to ODF2 and protamines are marked. Bands containing predominantly PRM1 and mature PRM2 (mP2) are labelled and areas of bands containing Prm2 precursors are marked (red dashed box). Non-protamine, nuclear-enriched proteins are found to be differentially abundant in dHET compared to WT sperm (red box). (D) Upper part of image shown in (C) compared to merged Western Blots against ODF2, histone H3 and histone H4 (pan-H3 and pan-H4 antibodies). (E) Western blots against histone H3 and H4 basic protein extractions of WT testis lysate and sperm samples form WT, dHET, Prm1 +/- (P1 +/- ), Prm1 -/- (P1 -/- ), Prm2 +/- (P2 +/- ), Prm2 -/- (P2 -/- ). ODF2 was used as loading control, as described before . (F) IHC against pre-PRM2 (antibody epitope in cP2) on WT and dHET epididymal caput tissue sections. (G) IHC against transition proteins TNP1 and TNP2 on WT and dHET epididymal caput tissue sections. Scales (F-G): 50 µm

Journal: bioRxiv

Article Title: Male mice heterozygous for Protamine-1 and Protamine-2 are infertile displaying sperm damage and retention of Protamine-2 precursors, transition proteins and histones

doi: 10.64898/2026.03.15.711850

Figure Lengend Snippet: (A) CMA3 staining of mature WT and dHET sperm counterstained by DAPI. Scale bars: 30 μm. (B) Quantification of CMA3-stained nuclei in WT and dHET samples. Data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (***p<0.001). (C) Representative image of a Coomassie stained AU-PAGE of basic nuclear-enriched proteins isolated from WT and dHET epididymal sperm. Bands corresponding to ODF2 and protamines are marked. Bands containing predominantly PRM1 and mature PRM2 (mP2) are labelled and areas of bands containing Prm2 precursors are marked (red dashed box). Non-protamine, nuclear-enriched proteins are found to be differentially abundant in dHET compared to WT sperm (red box). (D) Upper part of image shown in (C) compared to merged Western Blots against ODF2, histone H3 and histone H4 (pan-H3 and pan-H4 antibodies). (E) Western blots against histone H3 and H4 basic protein extractions of WT testis lysate and sperm samples form WT, dHET, Prm1 +/- (P1 +/- ), Prm1 -/- (P1 -/- ), Prm2 +/- (P2 +/- ), Prm2 -/- (P2 -/- ). ODF2 was used as loading control, as described before . (F) IHC against pre-PRM2 (antibody epitope in cP2) on WT and dHET epididymal caput tissue sections. (G) IHC against transition proteins TNP1 and TNP2 on WT and dHET epididymal caput tissue sections. Scales (F-G): 50 µm

Article Snippet: Primary antibodies diluted in blocking solution were added to the membrane and incubated overnight at 4 °C [PRM1 (Briar Patch Biosciences; Mab-Hup1N-150; 1:1000), PRM2 (Briar Patch Biosciences; Mab-Hup2B-150; 1:1000), ODF2 (proteintech; 12058-1-AP; 1:500-1:1000), histone H3 (abcam; ab1791; 1:1000), histone H4 (abcam; ab177840 ; 1:500)].

Techniques: Staining, Two Tailed Test, Isolation, Western Blot, Control